pGLO Lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 76 76 0 - pGLO LB/amp 0 0 0 + pGLO LB/amp 0 0 0 + pGLO LB/amp/ara 13 13 13

2.	What two new traits do your transformed bacteria have?
	The transformed bacteria have the 2 new traits of glowing under UV light and ampicillin resistance.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	I estimate that the amount of bacteria inside the 100 uL was roughly the same amount as the amount of colonies there where. This would be because once a bacteria landed on a certain spot it would multiply exponentially and create an entire colony.
4.	What is the role of arabinose in the plates?
	The role of arabinose was to create a nutrient rich environment for the glowing bacteria so it could have all the nutrients needed for a bright glow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	Tagging certain genes so their expression in certain cells will be known. Acting as a biosensor or cell marker when studying protein-protein interactions. visualizing promoter activity.
6.	Give an example of another application of genetic engineering. Other kinds of plasmids could be used to change certain genes and gene expressions in order to make organisms that produce certain kinds of proteins that can have benefits to health or sustainability. For example a plasmid could be used in bacteria to create a foe meat or other kind of food.